Effects of insulin on proliferation and apoptosis of endometrial carcinoma cell
- VernacularTitle:胰岛素对子宫内膜癌细胞增殖和凋亡的影响
- Author:
Jing ZHAO
;
Feng-Xia XUE
;
Shao-Fang HUA
;
Li-Zhi ZHANG
;
- Publication Type:Journal Article
- Keywords:
Endometrial neoplasms;
Insulin;
Receptor;
insulin;
Cell division;
Apoptosis
- From:
Chinese Journal of Obstetrics and Gynecology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of insulin receptor(INSR)in endometrial cell line Ishikawa3-H-12,and the effects of insulin on proliferation,cell cycle distribution and apoptosis of Ishikawa3-H-12 cells.Methods Immunocytochemistry and RT-PCR methods were used to investigate the expression of INSR in Ishikawa3-H-12 cells.The effects of insulin at different concentrations and different time on proliferation,apoptosis and cell cycle distribution of endometrial carcinoma cells were observed by methyl thiazolyl tetrazolium(MTT)assay and fluorescence-activated cell sorting technique.Results(1) We demonstrated the expression of INSR in Ishikawa3-H-12 cell line.(2)Incubation with insulin stimulated a dose- and time-dependent proliferation response in Ishikawa3-H-12 ceils with the peak response occurring at 48 hours with 1?10~(-4)mol/L insulin incubation[proliferative rate:(340.2?15.9)%,vs control (100%),P
