Spindle and chromosome configuration of human in vitro matured oocytes after slow freezing-fast thawing
- VernacularTitle:冷冻保存人类未成熟卵母细胞对其发育潜能的影响
- Author:
Shan-Shan GAO
;
Yuan LI
;
Zi-Jiang CHEN
;
Mei LI
;
Jing-Mei HU
;
Shui-Ying MA
;
- Publication Type:Journal Article
- Keywords:
Oocytes;
Cryopreservation;
Mitotic spindle apparatus
- From:
Chinese Journal of Obstetrics and Gynecology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the development of immature oocytes after freezing-thawing by conventional cryopreservation method for mature oocytes.Methods Immature oocytes were collected from stimulated ovaries of intracytoplasmic sperm injection(ICSI)cycles.Immature oocytes were in vitro matured directly or after slow freezing-fast thawing and immunostained for tubulin and chromatin and at last visualized by confocal microscopy.Results No statistical difference was found in maturity rate between freezing groups and the controls.There was a statistically significant increase in abnormalities of chromosome(23.7% vs. 50%)and spindle(28.9% vs.53.9%)in the GV freezing group compared with the GV control(P
