The role of the activation and expression of signal transducer and activator of transcription-6 in patients with ulcerative colitis
- VernacularTitle:溃疡性结肠炎中信号转导和转录活化因子6的活化表达及作用
- Author:
Yan-Hua PANG
;
Jian-Yu HAO
;
Wen-Jie ZHANG
;
Changqing ZHENG
;
- Publication Type:Journal Article
- Keywords:
Colitis,ulcerative;
Signal transduction;
Transactivators
- From:
Chinese Journal of Digestion
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the pathogenesis of the expression and activation of signal transducers and activator of transcription(STAT) 6 in patients with ulcerative colitis(UC).Methods Thirty patients with active UC(diagnosed with colonoscopy with pathologicaly conformation),who were not treated with steroids or immunomodulators,and 30 age-matched healthy controls were studied.The expressions of phosphorylated STAT6 and non-phosphorylated STAT6 in nucleus and cytoplasm were deterimined by Western blot.DNA binding activity of STAT6 was tested by electrophoresis mobility shift assay(EMSA).Results The expression of phosphorylated STAT6 was higher in nucleus than that in cytoplasm in mild UC patients,but the expression was higher in cytoplasm than that in nucleus in moderate to sever UC patients (P
