Effect of vascular endothelial growth factor on synthesis of pulmonary surfactant
- VernacularTitle:血管内皮生长因子对肺表面活性物质合成的影响
- Author:
Tao LI
;
Yu XIAO
;
Hong-Wei WANG
;
Gen-Rong MAI
;
Shao-Yong XU
;
Ji-Jian XIE
;
Dong-Sheng LI
;
- Publication Type:Journal Article
- Keywords:
Phospholipids;
Vascular endothehal growth factors;
Pulmonary surfactant associated proteins
- From:
Chinese Journal of Emergency Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of vascular endothelial growth factor(VEGF)on production of pulmonary surfactants.Method Fetal rat lungs were obtained at 19-day gestation.Primary culture of typeⅡalveolar epithelial cells(AECⅡ)was performed using IgG panning technique.The rats was divided into groups: VEGF,Dexamethasone,VEGF plus Dexamethasone and a control.Total phospholipids,phosphatidylcholine (PC),phosphatidyl glycerol(PG)and sphingornyelin(SM)were determined.Results expressed as mean?SEM. Comparison between groups were made with LSD-t test and one -way ANOVA.Result VEGF,Dexamethasone and VEGF plus Dexamethasone groups showed increased amount of total phospholipids and its compositions on the first day of culture.Conclusions VEGF-165 promotes the production and secretion of pulmonary surfactant. VEGF and Dexamethason may go through different mechanism for enhancement of synthesis of pulmonary surfactant,thereby improve biological function of AECⅡ.
