Establishment of experimental subarachnoid hemorrhage model with endo-cerebrovascular perforation
- VernacularTitle:脑血管穿破法建立大鼠蛛网膜下腔出血模型
- Author:
Jie-Sheng ZHENG
;
Yue-Hui MA
;
Ren-Ya ZHAN
;
Yong-Qin ZHOU
;
- Publication Type:Journal Article
- Keywords:
Subarachnoid hemorrhage;
Animal model;
Cerebrovascular spasm
- From:
Chinese Journal of Emergency Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an experimental subarachnoid hemorrhage(SAH)model with endo- cerebrovascular peroration.Method The right external carotid artery of SD rats were isolated,leaving a stump of approximately 3 to 4 mm.A-3-O monofilament nylon suture was inserted up through the stump of external carotid artery to the internal carotid artery for about 18～19 mm.A small resistance was usually felt,and the suture was then advanced 2 mm further and the suture was immediately withdrawn.Two hours or two days after SAH induction,SAH extension was observed.Two days after SAH induction,the diameter of the basilar artery was measured.Results SAH extends from the ipsilateral artery to the eontralateral artery after SAH induction.The diameters of basilar arteries in SAH animals were smaller than those of control rats,indicating the present of cerebrovascular spasm in SAH animals.Conclusions The endo-cerebrovascular perforation technique for establishing a non-craniotomy SAH model is reliable.
