Reversal of drug resistance in human ovarian cancer cells by wild-type PTEN gene and its mechanisms
- VernacularTitle:PTEN基因逆转卵巢上皮性癌细胞耐药的机制研究
- Author:
Hui-Juan WU
;
Hai-Tao WU
;
Dan-Hui WENG
;
Hui XING
;
Yun-Ping LU
;
Ding MA
;
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
PTEN phosphohydrolase;
Cisplatin;
Drug resistance;
neoplasm
- From:
Chinese Journal of Obstetrics and Gynecology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine expression of PTEN gene in ovarian cancer cisplatin-sensitive cell line OV2008 cells and cisplatin-resistant cell line C13K cells,and evaluate the effect of wild-type PTEN gene on reversing cisplatin-resistance of C13K cells and underlying mechanisms.Methods The expression of PTEN mRNA and protein in OV2008 and C13K cells were detected by semi-quantitative RT-PCR and western blot.Recombinant eukaryotic expression plasmid containing human wild-type PTEN gene was transfected into C13K cells by lipofectamine 2000.The expression of PTEN mRNA was monitored by RT- PCR and the expression of PTEN,protein kinase B(AKT),phospho-AKT(p-AKT)protein were analyzed by western blot in PTEN transfected and untransfected C13K cells.Proliferation and chemosensitivity of cells to cisplatin were measured by methyl thiazolyl tetrazolium(MTT),and cell apoptosis was detected by flow cytometry after treatment with cisplatin.Results(1)The expression of PTEN mRNA and protein(1.02 ?0.05,1.02?0.07)in OV2008 cells were significantly higher than those in C13K cells,which were 0.45 ?0.03 and 0.55?0.03 respectively(P
