Effects of nuclear factor-?B on cell apoptosis and left ventricular segmental function in acute myocardial ischemia-reperfusion
- VernacularTitle:核因子-?B对急性缺血-再灌流心肌细胞凋亡及左室局部功能的影响
- Author:
Ping LIN
;
Wei-Dong REN
;
Zhao-Hui WANG
;
Chang-Hong LIU
;
Jun WU
;
Yue-Ai WANG
;
- Publication Type:Journal Article
- Keywords:
NF-?B;
Myocardium;
lschemia-reperfusion;
Apoptosis
- From:
Chinese Journal of Emergency Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To probe the effects of nuclear factor kappa B (NF-?B) on cell apoptosis and left ventricular segmental function in acute ischemia repeffusion in dogs.Method Twenty-four dogs were randomly divided into three groups:without left anterior artery (lAD) ligation group (C group),LAD was occluded 30 min following reperfusion 120 minutes in isehemical reperfusion group (IR group),and dogs were administered with PDTC before LAD ligation in ischemical reperfusion plus pyorrole dithitocarbamate group (PDTC group).The left ventricular segmental function was detected by echo cardiography using strain rate anlysis software.EF measured by Simpson's method.Cardiac myocyte apoptosis numbers were determined by terminal deoxynudeotidy transferease-mediated biotinylated deoxyuridine triphosphate nick end labeling (TUNEL).lmmunohistochemistry and western-blot anylysis of NF-?B protein expression.Results NF-?B was obviously expression on injury myocardium of IR group,and increased significantly in contrast to control group (P0.05)Conclusions NF-?B might play an important role in acute myocardial ischemia reperfusion.PDTC reduces myocardial iscbemia/repeffasion injury by preventing expression of factor NF-?B.
