mRNA expression change of myelin gene in hippocampus following cerebral ischemia
- VernacularTitle:脑缺血后髓鞘基因表达变化的规律
- Author:
Ying-Zhu CHEN
;
Huan BAO
;
Ye TIAN
;
Shi-Yao BAO
;
Jun XU
;
Cheng-Lin YUAN
;
- Publication Type:Journal Article
- Keywords:
Myelin gene;
Cerebral ischemia;
Hippocampus;
RT-PCR
- From:
Chinese Journal of Emergency Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mRNA expression of myelin basic protein (MBP) and myelin oligodendregha glyeoprotein (MOG) in hippocampus of rats following global brain ischemia.Method The four- vessel occlusion animal model in the Sprague-Dawley rats was used in this study.The mRNA expression levels of MBP and MOG in the hippocampus of rats were analyzed by reverse transcription polymerase chain reaction (RT- PCR) at day 2,4,7,14 and 28 days after global brain ischemia.There were eight rats at each time-point and sham operated group.Results The mRNA expression of both MBP and MOG in hippocampus of rats decreased at 2 days after global brain ischemia.The gene expression of myelin gene decreased significantly at 7 days and it reached to the lowest level at 28 days.Compared with sham operated group,the gene expression of MBP and MOG in hippocampus of rats decreased significantly at 7,14 and 28 days after global brain ischemia (P
