IgA and Differentiation-associated Transcription Factors in Chronic Otitis Media with Effusion.
- Author:
Il Ho SHIN
1
;
Ok Young SHIN
;
Sung Ho CHA
;
Young Il KIM
;
Jin Woo LEE
;
Seung Geun YEO
Author Information
1. Department of Otorhinolaryngology, Kyung Hee University School of Medicine, Seoul, Korea. yeo2park@yahoo.co.kr
- Publication Type:Original Article
- Keywords:
Immunoglobulin;
Transcription factor;
Otitis media with effusion
- MeSH:
Antibodies;
Antibody Formation;
B-Lymphocytes;
Child;
Ear, Middle;
Enzyme-Linked Immunosorbent Assay;
Humans;
Immunoglobulin A;
Immunoglobulins;
Otitis;
Otitis Media;
Otitis Media with Effusion;
Recurrence;
RNA, Messenger;
Transcription Factors
- From:Clinical and Experimental Otorhinolaryngology
2009;2(3):131-135
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: Inadequate antibody responses to pathogens may lead to the recurrence of otitis media with effusion (OME). Although B-cell production by antibodies is controlled by transcription factors, the status of these factors has not been assessed in patients with OME. METHODS: Expression of immunoglobulin was measured by enzyme-linked immunosorbent assay. Expression of transcription factors Bcl-6, Blimp-1, Pax-5, and XBP-1 was assessed by RT-PCR in the middle-ear fluid of 29 children with >4 OME episodes in 12 months or >3 episodes in 6 months (the OME-prone group) and in 32 children with <3 OME episodes in 12 months (OME group). The relationship between recurrence of OME and expression levels of immunoglobulins and transcription factors in middle-ear fluid was determined. RESULTS: The concentration of IgA in middle-ear fluid was significantly lower in the OME-prone than in the OME group, as was the expression of mRNA encoding the transcription factors Blimp-1 and XBP-1 (P<0.05 each). Expression of mRNA encoding the transcription factors Bcl-6 and Pax-5 was more intense in the OME-prone than in the OME group, but these differences were not significant (P>0.05). CONCLUSION: Lower concentrations of IgA, Blimp-1 and XBP-1 in middle ear fluid of patients with OME may be related to OME recurrence and chronicity.
