Protective effect of penehyclidine hydrochloride in cerebral ischemia-reperfusion injury rats
- VernacularTitle:盐酸戊乙奎醚对大鼠脑缺血-再灌流损伤的保护作用
- Author:
Tie XU
;
Tao PANG
;
Xue-Mei ZONG
;
Gao-Yong LIANG
;
Zhi-Ping WANG
;
De-Qin GENG
;
Xian-Liang YAN
;
Hou-Qing WANG
;
- Publication Type:Journal Article
- Keywords:
Penehychdine hydrochloride;
Cerebral ischemia-reperfusion injury;
Cell apoptosis;
Bel-2;
Bax;
Rat
- From:
Chinese Journal of Emergency Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of a selective muscarinic receptor antagonist (penehyclidine hydrochloride) in three vessel occlusion model of acute global cerebral ischemia-reperfusion in rats.Method One hundred and forty-four male SD rats were randomly divided into four groups:sham operated group,vehicle treated group (saline 1 ml,i.p.),scopolamine treated group (0.01 mg/kg,i.p.) and penehyclidine hydrochloride treated group (0.01 mg/kg,i.p.) with drugs injected 40 minutes before ischemia respectively.The ischemic duration was 10 minutes.The animals were subjected to motor activity tests (open field activity test,beam-walking test and grip test) at 24 hours or on the 3rd and 7th day after reperfusion.HE staining,TUNEL staining and immunohistochemical reactions of bax and bel-2 were carried out at the time points of 2,12,24 hours,3 and 7 days after reperfusion.TTC staining was carried out in some rats for assessment of infarction volume on the 4th day after reperfusion.Results As compared with the vehicle treated group,both penehyclidine hydrochloride treatment and scopolamine treatment decreased the numbers of apeptotie neurons (P
