The effects of pulsed electromagnetic fields on proliferation and differentiation of endothelial progenitor cells
- VernacularTitle:脉冲电磁场对原代大鼠骨髓来源内皮前体细胞生长及分化的影响
- Author:
Fei LI
;
Kang CHENG
;
Hai-Chang WANG
;
Ke-Wei XU
;
Wen-Yi GUO
;
Rong-Qing ZHANG
;
- Publication Type:Journal Article
- Keywords:
Endothelial progenitor cells;
Proliferation;
Differentiation;
Pulsed electromagnetic fields
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of pulsed electromagnetic fields(PEMFs)on proliferation and differentiation of endothelial progenitor cells(EPCs).Methods EPCs were isolated from rat bone marrow by density gradient centrifugation.EPCs were exposed to PEMFs from the 5th day to the end of culture.MTT was used to measure the proliferation of EPCs.The expression ofⅧ-related antigen and NOS_3 was evaluated by flow cytometry. Results Compared with the control,the proliferating ability of EPCs exposed to PEMFs was stronger;the number ofⅧ-related antigen and NOS_3 positive cells increased significantly in EPCs exposed in PEMFs.Conclusion PEMFs promotes the proliferation and differentiation of rat bone marrow EPCs.
