Effects of ischemic postconditioning on myocardial cell during ischemia/reperfusion period in rabbits
- VernacularTitle:缺血后适应对兔短期缺血再灌注心肌细胞凋亡的影响
- Author:
Yu-Tao WEI
;
Jun-Feng DU
;
Jia-Long ZHU
;
Bo LUO
;
Zhijun ZHU
;
Yajun XUE
;
- Publication Type:Journal Article
- Keywords:
Myocardial reperfusion injury;
Apoptosis;
Ischemic postconditioning
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ischemic postconditioning (Post) on myocardial cell apoptosis and expres- sion of Bcl-2 and Bax protein during ischemia/reperfusion period in rabbits.Methods Eighteen rabbits were randomly allocated to three groups (6 in each group),sham operation (group S),ischemia/reperfusion group(group IR) and ischemic postconditioning group(group Post).Group IR and group Post were subjected to 15 minutes of left anterior descending coronary artery occlusion followed for 30 minutes of reperfusion.Ischemic postconditioning was achieved by three 30 seconds cycles of reperfusion,each followed by 30 seconds ischemia.Cardiomyocyte apoptosis were determined by in situ TDT-mediated dUTP nick end labeling (TUNEL) and DNA electrophoresis.The expression of Bcl-2 and Bax proteins in apoptotic myocardial cells were detected by immunohistochemistry sepa- rately.Results Compared with group IR,apoptotic index was significantly reduced in group Post [(28.06?2.92) % vs.(55.70? 13.96)%,P
