Effect of total glucosides of paeony on the proliferation of fibroblast-like synovial cells in osteoarthritis
- VernacularTitle:白芍总苷对骨关节炎成纤维样滑膜细胞的影响
- Author:
Qiang SHU
;
Xing-Fu LI
;
Dong LI
;
Huai-Shui HOU
;
Guo-Feng DAI
;
Hua-Xiang LIU
;
- Publication Type:Journal Article
- Keywords:
Osteoarthritis;
Fibroblast-like synovial cell;
Total glucosides of paeony
- From:
Chinese Journal of Rheumatology
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the proliferative characteristics of fibroblast-like synovial cells (FLS)in osteoarthritis in vitro and the mechanism of the immunosuppressive effect of total glucusides of paeony(TGP).Methods FLS of OA and non-inflamed synovium(NS)were cultured and identified in vitro in the presence or absence of TGP.After incubation,the survival fraction(SF)of FLS was evaluated by MTI' and the TNF-?,IFN-?and bFGF level in cultured FLS supernatant was measured by ELISA.The expression of FLS c-los mRNA and cell cycle of OA-FLS was observed by RT-PCR and flow eytometry respectively at the same time.Results No statistical significant differences were noted between the OA and NS FLS in pro- liferating double time.High doses of TGP suppressed FLS-SF more evidently in OA patients than in NS(P0.05).Conclusion High dose TGP can inhibit OA-FLS proliferation,modulate cy- tokine secretion and c-fos expression in OA.This suggests that TGP has immunosuppressive effect on OA syn- ovitis,probably by preventing the synovial hypertrophy in OA.
