Effects of recombined rat insulin-like growth factor-1 gene with or without transforming growth factor beta-1 gene on osteoarthritis of rabbit knee in vivo
- VernacularTitle:双基因联合转染治疗兔膝骨关节炎的实验研究
- Author:
Chuan XIANG
;
Xiao-Chun WEI
;
Jingyuan DU
;
Xi-Sheng WENG
;
Peng-Cui LI
;
Juan DING
;
- Publication Type:Journal Article
- Keywords:
Chondrocytes;
Gene therapy;
Transfection;
Transforming growth factor beta;
Insulinlike growth factorⅠ
- From:
Chinese Journal of Rheumatology
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the therapeutic effect of recombined rat insulin-like growth factory 1 gene and transforming growth factor beta-1(TGF-?_1)gene on anterior cruciate ligament transection(ACLT)- induced osteoarthritis-like changes in NZW rabbit knee joints.Methods Eighteen NZW rabbits were divided into 3 groups randomly after osteoanhritis was established by ACLT and another six rabbits were used as normal control group(group 1).Chondrocytes which had been transfected with IGF-1 gene,co-transfected with TGF-?_1 and IGF-1 gene(group 3,4)were injected into the rabbits knee joints.Experimental control group(group 2)only had ACLT bul was not transfected.After 4,8 weeks,rabbits were sacrificed and their joints were evaluated by morphological grades,histological examination,in situ hybridization examination,immunohistochemistry exami- nation,and transmission electron microscopy examination(TEM).Results The morphological grades showed that the normal control group had a very significant difference with the experimental control group(P
