Construction and expression of recombinant cecropin B-binding site of luteinizing hormone releasing hormone gene and its anticancer function
- VernacularTitle:天蚕素B-黄体生成素释放激素结合部位重组基因的构建及其抑癌功能的研究
- Author:
Xiao-Yong LI
;
He-Lian LI
;
Gui-Ying ZHENG
;
- Publication Type:Journal Article
- Keywords:
Antibiotics;
peptide;
Gonadorelin;
Recombination;
genetic;
Receptors;
LHRH;
Baculoviridae;
Cell line;
tumor
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct and express recombinant cecropin B-binding site of luteinizing hormone releasing hormone(CB-LHRH')gene,and to evaluate the anticancer function of CB-LHRH' on human ovarian cancer cell line SKOV3 and human endometrial cancer cell line HEC-1B.Methods The sequence of the cDNA encoding CB-LHRH' was designed,artificially synthesized,verified by DNA sequence analysis and expressed by Bac-to-Bac baculovirus expression system.The expression of CB-LHRH' proteins were identified by western dot blot using rabbit polyclonal antibody against LHRH as the primary antibody.To determine the anticancer effects of the CB-LHRH' protein,ovarian cancer cell line SKOV3 and endometrial adenocarcinoma cell line HEC-1B were treated by different doses of the CB-LHRH' protein.Cell growth inhibition assay was performed using the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)5[(phenylamino) carbonyl]-2H-tetrazolium hydroxide(XTT)kit at different times,and cell morphologic changes were observed under the inverted microscope.Results The inhibitory rate of proliferation by CB-LHRH' increased with the increase of dose and time respectively:SKOV3 cell,from(5.03?0.08)% to(53.24 ?1.22)%;HEC-1B cell,from(5.13?0.37)% to(56.16?1.08)%.The inhibitory effect on HEC-1B cell was stronger than that on SKOV3 cell(P
