Establishment of human ovarian carcinoma cell lines with directional highly lymphatic metastasis and study of their biological characteristics
- VernacularTitle:卵巢上皮性癌淋巴道定向高转移细胞系的建立及其生物学特性的鉴定
- Author:
He-Yun RUAN
;
Dan-Rong LI
;
Li LI
;
Xiao GUAN
;
Wei ZHANG
;
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
Lymphatic metastasis;
Cell line;
tumor;
Mice;
nude
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a human ovarian carcinoma cell line with directional highly lymphatic metastasis and to study their biological characteristics.Methods The clone cells of ovarian carcinoma,SKOV3,were inoculated into the hind foot pad of nude mice.The cancer cells of lymph node metastatic foci were transplanted into nude mice again when the metastatic nude of mice were observed.After repetition of this procedure for 3 cycles,the metastatic rate and the metastatic paths were observed in nude mice of every passage.We used limited dilution method to separate and select colonial cells with directional highly lymphatic metastatic potentials from the lymphatic metastasis of human ovarian carcinoma cell line SKOV3.The ceils with biological characteristics were assayed by growth curve,HE staining,karyotype analysis,nude mice transplantation and immunohistochemistry,respectively.Results We established a series of cell lines from lymph node metastasis and designated them as SKOV3-PM1,SKOV3-PM2 and SKOV3-PM3 cell strain.When the cells of SKOV3-PM3 were injected into the hind foot pad of nude mice, they produced 100%(10/10)spontaneous lymphatic metastasis.The lymphatic metastatic rates(26/10) were stable and higher than the mother cell line(1/10,P
