Detection of anti-M2 autoantibody using recombinant BCOADC-E2
- VernacularTitle:采用重组侧链二氧酸脱氢酶复合物E_2亚单位检测M_2抗体及其临床意义
- Author:
Yan CHEN
;
Ding-Kang YAO
;
Ye ZHOU
;
Ye ZHU
;
Ting-Wang JIANG
;
An-Mei DENG
;
Ren-Qian ZHONG
;
- Publication Type:Journal Article
- Keywords:
Liver cirrhosis,biliary;
Autoantibodies;
Immunoblotting;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Rheumatology
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect anti-M_2 autoantibody using recombinant BCOADC-E_2.Methods We purified recombinant BCOADC-E_2 by Ni~(2+)affinity chromatography column and then detect anti-M_2 autoan- tibody in the sera of patients with primary biliary cirrhosis(PBC)by Western blot test(WBT)and enzyme linked immunosorbent assays(ELISA).Results Among 60 sera from PBC patients,33 were positive,all of controls were negative.Conclusion The recombinant BCOADC-E_2 can be used to detect anti-M_2 autoanti- body specifically and sensitively.It is helpful for the diagnosis of PBC.
