Apoptosis and Expression of AQP5 and TGF-beta in the Irradiated Rat Submandibular Gland.
- Author:
Jin Hwa CHOI
1
;
Hong Gyun WU
;
Kyeong Cheon JUNG
;
Seung Hee LEE
;
Eun Kyung KWON
Author Information
1. Department of Radiation Oncology, Chung-Ang University Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Submandibular gland;
Radiation;
Apoptosis;
AQP5;
TGF-beta
- MeSH:
Acinar Cells;
Animals;
Apoptosis;
Aquaporin 5;
DNA Nucleotidylexotransferase;
Humans;
Male;
Rats;
Rats, Sprague-Dawley;
Submandibular Gland;
Transforming Growth Factor beta
- From:Cancer Research and Treatment
2009;41(3):145-154
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To evaluate the effect of X-ray irradiation on apoptosis and change of expression of aquaporin 5 (AQP5) and transforming growth factor-beta(TGF-beta) in the rat submandibular gland (SMG). MATERIALS AND METHODS: SMGs of 120 male Sprague-Dawley rats were irradiated with a single X-ray dose (3, 10, 20, or 30 Gy). At the early and late post-irradiation phase, apoptosis was measured by the terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) method, and expression of AQP5 and TGF-beta was determined by immunohistochemical staining. RESULTS: At the late post-irradiation phase, increased apoptosis was evident and marked decreases of expression of AQP5 expression by acinar cells and TGF-beta expression by ductal cells were evident. CONCLUSION: Apoptosis after X-ray irradiation develops relatively late in rat SMG. Irradiation reduces AQP5 and TGF-beta expression in different SMG cell types.
