Inhibitory effect of erythromycin derivative on the expression of NF-KB in active T lymphocytes
- VernacularTitle:红霉素衍生物抑制活化T淋巴细胞核转录因子-?B表达的研究
- Author:
Lan WU
;
Wei-Ge ZHANG
;
Ying-Wei QU
;
Li-Juan ZHAO
;
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
T-Lymphocytes;
NF-kappa B;
Erythromycin
- From:
Chinese Journal of Rheumatology
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the effects of erythromycin derivative,9(S)-hydroxyerythromycin, on the expression of NF-?B in active T lymphocytes,and evaluate the potential role in the treatment of rheuma- toid arthritis(RA).Methods Jurkat T cells were exposed to 100 pmol/L tumor necrosis factor(TNF)-?with or without pretreatment with 3,10,30 or I00?g/ml of erythromycin or 9(S)-hydrnxyerythromycin 1 h at 37℃. The expression of NF-?B was measured by reverse transcription-polymerase chain reaction(RT-PCR)and Western blot.Results Erythromycin and 9(S)-hydroxyerythromycin decreased the expression of NF-?B mRNA and protein in a dose-related fashion.The treatments with erythromycin(100?g/ml)and 9(S)-hydrox- yerythromycin(100?g/ml)resulted in about 36%,45% reduction of NF-?B mRNA(P
