The relationship between apoptosis and neurological impairment in experimental intracerebral hemorrhage in rats
- VernacularTitle:实验性脑出血后细胞凋亡与神经损伤的关系
- Author:
Hong-Ling LI
;
Qing-Rong ZHAO
;
Yu-Miao ZHANG
;
- Publication Type:Journal Article
- Keywords:
ICH;
Apoptosis;
Neurological impairment;
Rats;
Collagenase
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the apoptosis,expressions of Bcl-2 and Bax protein in perihematomal brain regions of rats and neurologic dysfunctions after intracerebral hemorrhage (ICH). Methods Seventy Sprague-Dawley rats were divided randomly into two groups:an experimental group and a control group.A model of ICH was established by injection of 0.5 U bacterial collagenaseⅦinto the caudate nucleus in the rats.Neurological impairment was evaluated at 6 h,12 h,24 h,48 h,72 h,7 d and 14 d after 1CH,respectively, before the rats were sacrificed.TUNEL method was used to detect apoptosis,and SP method to detect expressions of Bcl-2 and Bax protein in the perihematomal brain tissues.Results Neurological impairment occurred in all the rats after ICH,and peaked at 48 h after ICH.The apoptosis and expressions of Bcl-2 and Bax protein were peaked at 48 h,6 h and 48 h after ICH,respectively.Conclusion The degree of the neurological impairment after ICH is parallel to that of the apoptosis.Apoptosis may play an important role in neurological impairment after ICH.
