Low frequency transcranial magnetic stimulation changes seizure and neuropeptide Y expression of hippocampal neurons in rats epilepsy induced by pilocarpine
- VernacularTitle:低频经颅磁刺激对匹罗卡品致痫大鼠癫痫发作及海马细胞神经肽Y表达的影响
- Author:
Yong-Li WANG
;
Xiao-Lin HUO
;
Jian-Ning ZHANG
;
Zhaiyu
;
- Publication Type:Journal Article
- Keywords:
Low frequency transcranial magnetic stimulation;
Epilepsy;
Hippocampus;
Neuropeptide-Y
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of low frequency transcranial magnetic stimulation (LF-TMS) on the electroencephalogram (EEG),expression of NPY in hippocampus in pilocarpine (PLO)-induced epileptic rats. Methods Forty male Sprague-Dawley rats (240-260 g) were used to establish a model of epilepsy by in- tradominal injection of pilocarpine,and then randomized into 2 groups:a control group and an intervention group. The control group was treated by sham LF-TMS,while the intervention group was treated by LF-TMS once daily for 7 days.Ⅰgroup simply celiac inject pilocarpine.Ⅱgroup celiac inject PLO after LF-TMS.The EEG was recorded in both groups and the checked pathology.Pathological item include HE staining,NPY immunohisto chemical staining. Results The latency for seizure attack was significantly lengthened,while the frequency of seizure attack and times of major seizure attack were significantly decreased in the intervention group.The HE staining revealed significant de- generation and necrosis of neurons in the hippocampus,especially in the CA3 region,in rats in the control group. The pathologic changes were significantly less severe in the intervention,Immunohistochemical staining showed a sig- nificantly higher expression of NPY in the hippocampus as compared with the intervention group. Conclusion U- sing the PLO-induced epilepsy model,LF-TMS could not only postpone the generation of kindling but also inhibit the progress of epilepsy.The increased NPY expression in the hippocampusin the intervention group implied a close rela- tionship between NPY and epilepsy attack.
