Overexpression of estrogen receptor-related receptor a can stimulate estrogen receptor negative endometrial cancer cell proliferation
- VernacularTitle:雌激素受体相关受体?过度表达对雌激素受体阴性的子宫内膜癌细胞增殖的影响
- Author:
Peng-Ming SUN
;
Li-Hui WEI
;
Min GAO
;
Jian-Liu WANG
;
Li-Jun ZHAO
;
Da-Peng WANG
;
Jun-Xiao ZHANG
;
- Publication Type:Journal Article
- Keywords:
Endometrial neoplasms;
Receptor;
estrogen;
Cell division
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of human estrogen receptor-related receptor(ERR) ?,a submember of orphan receptors,in the tumorigenesis of endometrial cancer.Methods Plasmid of pSG-ERR? was transfected into endometrial cancer cell lines HEC-1A,HEC-1B,and Ishikawa.Real-time quantitative RT-PCR and western blot were used to analyze the mRNA and protein expression of ERR? in endometrial cancer cell.Flow cytometry was used to analyze the cellular growth.Results Expressions of the ERR? were significantly increased in the endometrial cancer cells transfected with pSG-ERR? plasmid; expression of the ERR? mRNA in HEC-1A cell was 9644.4 copies/ng,HEC-1B:9835.3 copies/ng,and Ishikawa:8008.6 copies/ng(P
