Human eukaryotic translation elongation factor 1 alpha 2 suppresses apoptosis in pancreatic cancer
- VernacularTitle:人类真核翻译延长因子1A2在胰腺癌中抑制凋亡致癌机制研究
- Author:
Qi ZHU
;
Su ZHANG
;
Hai-Xia CAO
;
Ju CAI
;
Yaobo GAO
;
Yongping ZHANG
;
Kai XU
;
Chong QI
;
- Publication Type:Journal Article
- Keywords:
Transcriptional elongation factors;
Pancreatic neoplasms;
RNA interference;
Apoptosis
- From:
Chinese Journal of Digestion
1998;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the potential mechanisms of carcinogenesis for human eukaryotic translation elongation factor 1 alpha 2(EEF1A2).Methods Specific inhibition of EEF1A2 with siRNA was achieved in human pancreatic cancer cell line,BxPC-3,which usually expresses high level of EEF1A2.The changes of EEF1A2 expression were determined by Western blot.The effect of siRNA in suppressing the proliferation of BxPC-3 cells was determined by MTT assay,and its role in inducing BxPC-3 cell apoptosis evaluated by flow cytometry,TUNEL and transmission electron micro- scope.Results The sequence-specific siRNA effectively suppressed the expression of both EEF1A2 mRNA and protein.Specific inhibition of EEF1A2 with siRNA in pancreatic cancer cell line BxPC-3 could suppress proliferation and induce apoptosis.Conclusion The oncogenicity of EEF1A2 may be related to its role in suppressing the apoptosis and promoting the growth of pancreatic cancer cells.
