Immunoregulatory effects of bone marrow-derived mesenchymal stem cells from MRL/Ipr mice on T lymphocytes
- VernacularTitle:MRL/lpr鼠骨髓间充质干细胞对T淋巴细胞作用的研究
- Author:
Hui LI
;
Ling-Yun SUN
;
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
T-lymphocytes;
Apoptosis;
Mice,inbred MRL/lpr
- From:
Chinese Journal of Rheumatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the biological characteristics and to compare the different regulation mechanisms of bone marrow mesenchymal stem cells(MSCs)derived from both lupus(MRL/Ipr)and normal (C57BL/6)mice on T iymphoeytes in vitro. Methods MSCs from MRL/lpr and C57BL/6 mice bone marrow were isolated and cultured. The surface phenotypes were detected by flow cytometry(FCM). The morphologi- cal changes of MSCs were evaluated in primary and passage cultures. The growth curves were assayed. CD3~+T lymphocytes from spleen of C57BL/6 mice were isolated by nylon wool columns and stimulated by ConA and co-cultured with or without the two strains of MSCs or supernatant for 72 h. The proliferation of T cells stained by CFSE and activation of T cells were detected. The apoptosis was assessed by now cytometry using Annexin V/PI. Results The growth of lupus MSCs was faster than normal controls(P
