The preliminary investigation of tumor necrosis factor-a blocker treatment on CD4~+CD25~+ Treg cells (peripheral blood)in ankylosing spondylitis patients
- VernacularTitle:抗肿瘤坏死因子-?治疗对强直性脊柱炎患者外周血CD4~+CD25~+T细胞调节作用的初步研究
- Author:
Hui-Qin HAO
;
Feng HUANG
;
Xiao-Hu DENG
;
Ya-Mei ZHANG
;
Jie TANG
;
Li-Ping PANG
;
Xian-Feng FANG
;
- Publication Type:Journal Article
- Keywords:
Spondylitis, ankylosing;
Tumor necrosis factor;
CD4~+CD25~+ T regulatory cells
- From:
Chinese Journal of Rheumatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of CD4~+CD25~+ Treg cells on the pathogenesis of ankylos- ing spondylitis(AS), and to study the machanism of tumor necrosis factor(TNF)-?blocker on the treatment of AS by detecting the number of CD4~+D25~+ Treg cells before and after the treatment. Methods The diagno- sis of 10 AS patients was made based on the 1984 modified New York criteria. The patients received subcuta- neou injection of recombinant human tumor necrosis factor receptor-Fc fusion protein(rhTNFR-Fc)(etaner- cept)50 mg weekly for 8 weeks and 10 heathy subjects were enrolled for control. The mononuclear cells were isolated from peripheral blood in beth patients and controls. The number of CD4~+CD25~+ T cells and CD4~+ CD25~(high)T cells and the expression of CTLA-4. were detected by flow cytometry. Results The proportion of CD4~+CD25~+ T cells(24?19)% in total CD4~+ T lymphocytes of peripheral blood and CD4~+CD25~(high)T/CD4~+ T (6?6)% from AS patients before treated with rhTNFR-Fc was higher than that in healthy volunteers and AS patients after treatment(P
