Effects of tanshinoneⅡ on the expression of c-fos and c-jun in angiotensin Ⅱ-induced hypertrophy of cardiomyocytes
- VernacularTitle:丹参酮ⅡA对血管紧张素Ⅱ诱导的心肌肥大c-fos和c-jun mRNA表达的影响
- Author:
Zhi ZHENG
;
Qian-Sheng LIANG
;
Jun FENG
;
- Publication Type:Journal Article
- Keywords:
AngiotensinⅡ;
TanshinoneⅡA;
Cardiomyocyte hypertrophy;
c-fos;
c-jun
- From:
Chinese Journal of Emergency Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of TanshinoneⅡA (TSN) on the cell hypertrophy induced by angiotensinⅡ(AngⅡ) in the primary culture of neonatal rat cardiomyocytes. Method The effect of TSN on cardiomyocyte was evaluated by the 3-[4, 5-dimethylthiazol-2-yl] -3, 5-diphenylformazan (MTF) assay. As the index of eardiomyocyte hypertrophy, protein synthesis rate was measured by H-Leucine incorporation and the cell size was determined by phase contrast microscope. The proto-oncogene c-los mRNA and c-jun mRNA expression was assessed using reverse transcription polymerase chain reaction (RT-PCR). Results Exposure of the myocytes to TSN (5～80 mmol/L) for 24hours produced no cytotoxicity. Protein synthesis rate and proto-oneogene c-fos and c-Jun mRNA expression of eardinmyoeytes increased significantly after AngⅡtreatment, and TSN inhibited these effect of AngⅡ.Conclusions TSN can prevent the hypertrophy of eardiomyocytes induced by AngⅡ, which be attributable relate to the decreased expression of proto-oncogene c-los and c-jun mRNA by TSN.
