Effect of propofol on nNOS expression after focal cerebral ischemia-reperfusion injury in rats
- VernacularTitle:异丙酚对大鼠局灶性脑缺血-再灌注损伤后nNOS表达的影响
- Author:
Si-Hua QI
;
Zi-Wei XIAN
;
Wen-Zhi LI
;
Bing ZHANG
;
Jun LI
;
Bao-Qing HAN
;
- Publication Type:Journal Article
- Keywords:
Propofol;
Brain ischemia;
Reperfusion injury;
Neural Nitric-oxide synthase
- From:
Chinese Journal of Emergency Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of propofol on nNOS expression after focal cerebral ischemia-reperfusion in rats and the possible mechanism of protective effect of propofol on brain. Method Seventy-eight male Wistar rats, weighting 250 ~ 300 g, were randomly divided into 3 groups:(1)Sham operation group (S group, n=6) was performed with scham operation; (2) Ischemia-reperfusion group (group I-R, n=36) was subjected to 2-hour right middle cerebral artery occlusion and then reperfusion was followed, saline (1 mg/kg) was injected into the right lateral cerebral ventricle using microsyringe before reperfusion;(3) Propefol group (group P, n=36) was injected with propofol (1mg/kg) into the right lateral cerebral ventricle using microsyringe right after ischemia. Group I-R and group P were divided into 3 subgroups according to the reperfusion time: 1 h, 3 h and 6 h. The neurological function of all rats were tested before reperfusion. The cerebral infarction area of the whole brain was calculated with TIC staining (n=6). The pathological change of brain was observed from HE staining (n=6) and the nNOS protein expression was obtained by immuno- histochemical method (n=6). Results Compared with I-R group, the neurological function was better in group P(P
