Insulinllike growth factor 2 imprinting status and promoter usage in the placenta of macrosomia
- VernacularTitle:胰岛素样生长因子2基因印迹状态及其启动子活性在巨大儿胎盘组织中的变化
- Author:
Jin-Cui YAO
;
Ya-Li HU
;
Zhi-Qun WANG
;
Yi-Min DAI
;
Jing-Xian LING
;
Xiao-Dong YE
;
- Publication Type:Journal Article
- Keywords:
Fetal macrosomia;
Placenta;
Insulin-like growth factor Ⅱ;
Genomic imprinting;
Promoter regions(genetics)
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of marcosomia by investigating insulin-like growth factor 2(IGF_2)imprinting status,expression level and the promoter usage in the placenta of macrosomia. Methods We selected heterozygous cases for Apa Ⅰ polymorphism in exon 9 of IGF_2 gene and then analyzed its imprinting status in 168 placentas of macrosomia and normal pregnancies.IGF_2 transcription levels and promoter usages in macrosomic and normal placenta were evaluated by using semi-quantitative RT- PCR assay.Results Thirty specimens of macrosomic placenta and 30 of normal placenta were identified as heterozygous for IGF_2.All of the heterozygous specimens showed maintenance of imprinting.The expression of placental IGF_2 mRNA(2.2?1.2)was significantly higher in macrosomia than that of normal weight group (1.6?0.6,P 0.05).Conclusion It is possible that over expression of IGF_2 in placenta contributes to macrosomia while the promoter usage and imprinting status are not associated with macrosomia.
