Observation of Differential Protein of Hypothalamus in the Hyperthyroidlsm Rats with Hyperactivity of Liver-Yang
- VernacularTitle:甲状腺功能亢进肝阳上亢证大鼠下丘脑组织蛋白质差异表达的观察
- Author:
Ling-Yan ZHOU
;
Ze-Qi CHEN
;
Wei LI
;
- Publication Type:Journal Article
- Keywords:
hyperthyroidism;
liver-yang hyperactivity;
hypothalamus;
Proteome;
animal model
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Utilization of proteome method,by observing differences on protein expression of hypothalamus in the hyperthyroidism rats with hyperactivity of liver-yang and normal rats,to search the differential protein.Methods Models of animal were prepared by ip L-T4 and Fuzi Decoction.Both the quantitative and qualitative changes of the protein expression were compared between model group and treated group by protein technique.Results Proteins were mainly displayed at range of pI 3～8,Mr(14.4～75)kD.18 protein increased and 24 protein decreased in model group compared with treated group, included Thioredoxin,Ubiquitin carboxyl-terminal hydrolase isozyme L1,Platelet-activating factor acetylhydrolase IB gamma subunit,glutamate dehydrogenase,dihydropyrimidinase-related protein 2, NSFL1 cofactor p47,Tubulin beta 5.Conc(?)sion There were 18 spots high expressed and 24 spots low expressed in the hyperthyroidism with hyperactivity of liver-yang,that maybe related with the develepment of the hyperthyroidism with hyperactivity of liver-yang.
