Determination of Oleanolic Acid and Ursolic Acid in the Fruits of Ligustrum Iucidum Ait.of Shanxi by HPLC
- VernacularTitle:HPLC法测定陕西女贞子中齐墩果酸与熊果酸的含量
- Author:
Zhi-Hua QI
;
Si-Wang WANG
;
- Publication Type:Journal Article
- Keywords:
Fructus Ligustri Lucidi;
Shanxi;
habitat;
growing stages;
oleanolic acid;
ursolic acid;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine oleanolic acid(OA)and ursolic acid(UA)from Fructus Ligustri Lucidi in diffrernt habitat and various growing stages.Method We picked the fruits of Fructus Ligustri Lucidi in Otober in five cities of Shanxi,and in August,September,October,November and December in Xi'an.Removed impurities and storaged the fruits under room temperature.By HPLC with Waters 600 as its chromatographic system,and Lichrospher C_(18)(4.6mmx250mm,5?m) column was applied with CH_3CN-CH_3OH-H_2O-H_3PO_4-(C_2H_5)_3N(50:30:20:0.02:0.04)as its mobile phase,the flow rate was 1 mL/min.The standard working curve was made to determine the contents of OA and UA at different habitat and different time spot from samples.Result The contents of OA and UA were highest in Ankang city.During prolonging growing stages,the highest contents of OA and UA were October and August,respectively.They both reduced to the lowest point in December.Conclusion The contents of OA and UA changed in different habitat and diffrernt growing stages of Fructus Ligustri Lucidi. It was suggested that we should mainly base on the highest contents to select the harvest time according to our demands.
