Effect of escharectomy on rats'pulmonary NF-?B activation in early stage of burn injury
- VernacularTitle:早期切痂对烧伤大鼠肺组织核因子-?B活化的影响
- Author:
Zhi-Qing LI
;
Yue-Sheng HUANG
;
Zong-Cheng YANG
;
Jia-Han WANG
;
- Publication Type:Journal Article
- Keywords:
Burns;
Lung;
Nuclear factor-?B
- From:
Chinese Journal of Emergency Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of escharectomy on rats' pulmonary NF-?B activation and the expression of pulmonary proinflammatory cytokines in early stage of burn injury.Method Wistar rats were randomly divided into three groups:group A(control group),group B(postburn without escharectomy),group C(escharectomy at early stage of burn injury).Thermal-injuried rats underwent 35% TBSA full-thickness burns. Activation of pulmonary NF-?B at 12 hours and 24 hours postburn was tested by electrophoretic mobility shift assay (EMSA),and at the same time expressions of pulmonary TNF-?mRNA were measured by reverse transcription polymerase chain reaction(RT-PCR)and release of pulmonary TNF-?were assayed by enzyme-linked immunosorbent assay(ELISA).Results Compared with control group,activity of pulmonary NF-?B in group B was markedly increased,reached(19.56?1.36)?10~4 A at 12 hours and(15.23?1.94)?10~4 A at 24 hours,which was higher than that in group A[(4.36?0.38)?10~4 A,P
