The effect of electromagnetic fields on gene expression profiles in bone marrow mesenchymal stem cells
- VernacularTitle:工频磁场对骨髓间充质干细胞基因表达谱的影响
- Author:
Hai-Jun ZHANG
;
Hua WU
;
Wei-Jun LIU
;
Hai-Hu HAO
;
Wen-Chun ZHAO
;
- Publication Type:Journal Article
- Keywords:
Electromagnetic fields;
Gene expression profiles;
Bone marrow mesenchymal stem cells;
Gene chips
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen for differential expression of genes in bone marrow mesenchymal stem ceils (MSCs) stimulated by electromagnetic fields (EMFs) and to study the mechanism of differentiation of the bone mar- row MSCs induced by EMFs.Methods Mouse bone marrow MSCs were isolated and cultured in vitro.The third- passage cells were stimulated by EMFs,then the total RNA was extracted,purified to cDNA and reversely transcribed to yield a cRNA probe.The cRNA probes from stimulated and control cells were labelled with Cy5 and Cy3,respec- tively.The two samples were hybridized with a mouse oligo microarray,and the hybridization signals were scanned. The reverse transcriptase polymerase chain reaction (RT-PCR) was used to identify the interesting genes:Duspl and Rabl.Results A total of 153 differentially expressed genes were found comparing the stimulated group and the control group.There were 74 up-regulated genes and 79 down-regulated genes in the stimulated group.Semi-quantita- tive RT-PCR confirmed that the Duspl and Rabl mRNA expression levels of the stimulated group were up-regulated. Conclusion The gene expression profiles of the bone marrow MSCs were altered after EMF exposure.The genes are involved in cell proliferation and differentiation,transcription control,metabolism and response to stress.These re- sults provide deeper insight into the mechanism of differentiation of the bone marrow MSCs.
