The influence of high power microwave irradiation on the expression of neuropeptide Y and neural nitric oxide synthase in the rat cerebral cortex and hippocampus
- VernacularTitle:高功率微波辐射对大鼠脑皮质及海马神经肽Y、nNOS表达的影响
- Author:
Li-Feng WANG
;
Xiang-Jun HU
;
Rui-Yun PENG
;
Xu WANG
;
Ya-Bing GAO
;
Jun-Jie MA
;
Shui-Ming WANG
;
Wen-Hua HU
;
De-Wen WANG
;
Zhentao SU
;
- Publication Type:Journal Article
- Keywords:
High power microwaves;
Cerebral cortex;
Hippocampus;
Neuropeptide;
Neural nitric oxide synthase
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of high power microwave(HPM) irradiation on neuropeptide Y (NPY) and neural nitric oxide synthase (nNOS) expression in the cerebral cortex and hippoeampus of Wistar rats. Methods A total of 110 Wistar rats were used for this study.Three groups of 30 Wistar rats were exposed to HPM irradiation at intensities of 3,10,30 and 100 mW/cm~2,respectively.Twenty rats served as controls and were ex- posed to sham HPM irradiation.At 6 h,and at 1,3,7,14 and 28 d after irradiation,five rats from each group were sacrificed,and their cerebral cortices and hippocampi were harvested.HE staining was used to highlight any change in the structure of the cerebral cortex or hippocampus.Immunohistochemistry techniques and image analysis were used to study the changes in NPY and nNOS expression.Results 10 to 100 mW/cm~2 HPM irradiation caused pyc- nosis and deep staining of some neurons in the cerebral cortex and hippocampus.The increase in nNOS expression and decrease in NPY expression observed were significant at 3 days after irradiation.Conclusion HPM irradiation can induce injury in neurons of the cerebral cortex and hippoeampus,and abnormal NPY and nNOS expression.
