Relationship between tyrosine phosphorylation and protein expression of insulin receptor substrate-1 and insulin resistance in gestational diabetes mellitus
- VernacularTitle:胰岛素受体底物1表达及其酪氨酸磷酸化与妊娠期糖尿病患者胰岛素抵抗的关系
- Author:
Hong-Mei QU
;
Yuan-Hua YE
;
Wei PENG
;
Ying ZHAN
;
- Publication Type:Journal Article
- Keywords:
Diabetes;
gestational;
Insulin resistance;
Tyrosine;
Phosphoproteins;
Phosphorylation
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between tyrosine phosphorylation(TP)and protein expression of insulin receptor substrate-1(IRS-1)and insulin resistance in patients with gestational diabetes mellitus(GDM).Methods IRS-1 expression and TP in skeleton muscle tissue were determined by Western blot and immunoprecipitation in women with GDM(GDM group,n=22),normal pregnant women(normal pregnancy group,n=22)and normal nonpregnant women(normal nonpregnant group,n=13).Fasting plasma glucose(FPG)and fasting insulin(FINS)were measured by oxidase assay and immunoradioassay. Results(1)The levels of FPG,FINS,and insulin resistance index were calculated according to homeostasis model assessment [ HOMA-IR;(5.6?0.8)mmol/L,(15.4?5.1)mU/L,and 1.2?0.5 ] in GDM group were significantly higher than those in normal pregnancy group [(4.4+0.5)mmol/L,(10.6 ?3.1)mU/L,and 0.8?0.3;P
