The expression and significance of glucose-6-phosphate isomerase mRNA in peripheral blood mononuclear cells of patients with rheumatoid arthritis
- VernacularTitle:葡萄糖-6-磷酸异构酶mRNA在RA患者外周血单个核细胞中的表达及意义
- Author:
Guan-Fei ZHAO
;
Yong-Zhe LI
;
Yu-Guo SONG
;
Chao-Jun HU
;
Da-Wei TONG
;
Shu-Lan ZHANG
;
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Polymerase chain reaction;
Glucose-6-phosphatc isomerase
- From:
Chinese Journal of Rheumatology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mRNA level of glucose-6-phosphate isomerase(GPI)ex- pression in patients with rheumatoid arthritis(RA).Method Reverse transcription-polymerase chain reaction (RT-PCR)was applied to semiquantitatively analyze GPI mRNA expression in the peripheral blood mononu- clear cells(PBMCs)of 30 active RA patients,30 stable RA patients,30 patients with other rheumatic disease and 30 healthy subjects.Results There was statistically significant differences between patients with RA and other rheumatic diseases(P
