Effect of rabbit adipose-derived stem cells transfected by adenoviral vector mediated hTGF-?_1 gene on chondrocyte differentiation in vitro
- VernacularTitle:腺病毒介导转染hTGF-?_1基因的兔脂肪干细胞向软骨细胞分化的实验研究
- Author:
Zhong FANG
;
Feng LI
;
Hong-Bo YOU
;
Wei XIONG
;
Guang-Hui LI
;
- Publication Type:Journal Article
- Keywords:
Genes;
Adipose-derived stem cell;
Chondrocytes;
Tissue engineering
- From:
Chinese Journal of Rheumatology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the proliferation of rabbit adipose-derived stem cell(ADSCs) transfected by adenoviral vector mediated hTGF-?_1 gene and its chondrocyte differentiation potential.Methods The Ad-hTGF-?_1 plasmid vetor which had the hTGF-?_1 gene was developed and transfected the ADSCs.The experimental group was the hTGF-?_1 transfected group.The cells enclosed by alginate were cultured in com- plete chondrogenie medium(CMM).The morphology of the cells were observed,and RT-PCR was used to measure hTGF-?_1 and collagenⅡexpression,at the same time western blot and immunohistochemistry were applied to detect the expression of collagenⅡin ADSCs before and after transfected with hTGF-?_1 gene. Results The hTGF-?_1 transfected ADSCs became the polygon and it proliferated well.The RT-PCR result of hTGF-?_1 on the transfected group was better than the control after transtected for 7 day and 21 day.The dif- ference between the two groups was significant(P
