HPLC Determination of Psoralen and Bergapten in Ficus hirta Vahl.
- VernacularTitle:HPLC法测定五指毛桃药材中补骨脂素和佛手柑内酯的含量
- Author:
Jingjin ZHONG
;
Zhanqiang LIU
;
Yuan'Er ZENG
;
Bin JIANG
;
Honghua XU
;
- Publication Type:Journal Article
- Keywords:
Ficus hirta Vahl.;
Content determination;
Psoralen;
Bergapten;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of psoralen and bergapten in Ficus hirta Vahl.Methods A Merck-lichrospher C18(4 mm?250 mm,5 ?m)column was adopted.The mobile phase was acetonitrile-water(35∶65) with the flow rate of 1.0 mL/min.The column temperature was 35 ℃,and the detection wavelength was set at 222 nm.Results Psoralen's linearity was obtained in the range of 0.12 ?g～1.20 ?g(r=0.999 7),and bergapten's linearity in the range of 0.03 ?g～0.30 ?g(r=0.999 5).Psoralen's average recovery was 100.9 %with RSD 2.9 %,and that of bergapten was 99.6 %with RSD 1.9 %.Conclusion This is the first report of simultaneous determination of psoralen and bergapten in Ficus hirta Vahl.by HPLC,and the results showed the method is accurate,reproducible,and can serve as quality evaluation method for Ficus hirta Vahl.
