The measurement of proportion and function of regulatory T cells in unexplained recurrent spontaneous abortion
- VernacularTitle:原因不明复发性流产患者调节性T淋巴细胞比例及功能的测定
- Author:
Yu-Sheng LIU
;
Shu-Yun ZHAO
;
Jing-Juan JI
;
Zhi-Min ZHAI
;
Xian- TONG
;
Li-Hua LUO
;
Hong-Bing LUAN
;
- Publication Type:Journal Article
- Keywords:
Abortion;
habitual;
T-lymphocytes
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the proportion and function of CD_4~+ CD_(25)~+ regulatory T cells (CD_4~+ CD_(25)~+ Tr)in unexplained recurrent spontaneous abortion(URSA).Methods(1)Proportion measurement:the proportion of CD_4~+ CD_(25)~+ Tr cells in peripheral blood was measured by double-label flow cytometric analysis.The samples were taken from 15 URSA women,15 normal non-pregnancy women and 13 normal pregnancy women.(2)Function measurement:CD_4~+ CD_(25)~+ Tr ceils and CD_4~+ CD_(25)~+ T ce]ls were extracted from peripheral blood lymphocytes by the microbeads separation.The purity of CD_4~+ CD_(25)~+ Tr cells and CD_4~+ CD_(25)~+ T cells was measured by flow cytometry.The growth inhibitory effect of CD_4~+ CD_(25)~+ Tr cells on CD_4~+ CD_(25)~+ T cells was assessed in vitro.Results The proportion of CD_4~+ CD_(25)~+ Tr cells was decreased significantly in URSA women(6.9?1.8)% than that in normal non-pregnancy women[(10.8?1.1)%] (P0.05).Conclusion The results suggest that decrease in proportion and function of CD_4~+ CD_(25)~+ Tr cells may be associated with URSA.
