Homocysteine decreases the invasion in cultured human trophoblasts:relationship between homocysteine and matrix metalloproteinase-2,-9 expression
- VernacularTitle:同型半胱氨酸对滋养细胞基质金属蛋白酶2、9表达的影响
- Author:
Pei-Feng YANG
;
Jin-Ke LI
;
Qing XIONG
;
- Publication Type:Journal Article
- Keywords:
Homocysteine;
Trophoblasts;
Pre-eclampsia;
Gelatinase A;
Gelatinase B
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test the hypothesis that homocysteine can decrease MMP-2 and MMP-9 expression in cultured trophoblasts of early pregnancy and that homocysteine can prevent trophoblasts invasion in the early stage of preeclampsia.Methods Cytotrophoblasts from early pregnancy were isolated and cultured.Trophoblasts were treated with or without Hcy(1 mmol/L)for 48 hour,and real time RT-PCR and gelatin zymography were used to quantify the mRNA and protease activity of MMP-2,-9.Results Treatment with Hcy(1 mmol/L)induced a decrease in MMP-2 mRNA by 21% and MMP-9 mRNA by 11%.At protein level MMP-2 expression decreased 14% and MMP-9 expression decreased 52% compared with control.Conclusions Homocysteine can decrease MMP-2,-9 expression in trophoblasts of early pregnancy and influence its invasion process.
