Investigation of transfection efficacy with transcatheter arterial transporting transferrin to enhance p53 gene
- VernacularTitle:经导管动脉输送转铁蛋白增强p53基因转染效率的研究
- Author:
Qin LU
;
Huan-Zhang NIU
;
Guang-Yu ZHU
;
Yan-Li AN
;
Ding-Hong QIU
;
Gao-Jun TENG
;
- Publication Type:Journal Article
- Keywords:
Transferrin;
Intervention;
p53;
Transfection efficiency;
Hepatic tumor
- From:
Journal of Interventional Radiology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the function of transferrin-DNA complex,transported by transferrin(Tf)and trans-arterial injection via interventional approach be the duel-target-orientated delivery and the transferring into malignant cells to get more effective therapy.Methods p53-LipofectAMINE ligand with different concentrations of Tf(0,10,25,50,100?g)transfected the 4 strains including LM6、Hep3B、YY and L02 in vitro to evaluate the gene transfeetion efficiency through western blot.Then,after setting up the VX2 hepatocarcinoma models,we delivered the Tf-p53-LipofeetAMINE complex into the hepatic arteries via interventional techniques to analyse the transfection efficiency in vivo.Results Tf,within the range of 10 100?g,could increase gene transfection efficiency mediated by liposome,and the efficiency increases with the raise of Tf concentration.Combination with interventional technique to inject Tf-DNA complex into tumor arteries,gene transfeetion efficiency was enhanced in rabbit models.Conclusion Tf can enhance gene- liposome transfection efficiency,furthermore with combination of interventional catheter technique,there would be a potential duel-target-orientated gene therapy method.(J Intervent Radiol,2007,16:99-103)
