Characterization of biophysical properties of insulin-producing cells labeled with SPIO-PLL in vitro
- VernacularTitle:诱导后大鼠胰岛素分泌细胞超顺磁化氧化铁颗粒-多聚左旋赖氨酸体外标记后生物活性研究
- Author:
Wei-Ying WANG
;
Gang DENG
;
Ai-Mei LI
;
Jun-Hui SUN
;
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cell;
Insulin-producing cell;
Magnetic resonance image;
Label
- From:
Journal of Interventional Radiology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the biophysical properties of insulin-producing cells labeled and unlabeled with superparamagnctic iron oxide nanoparticals and poly-L-lysine(SPIO-PLL)in vitro,and then monitor cellular imaging with 1.5 T MR.Methods BMSCs were isolated from tibia and femur of 6～8 weeks normal Spragne-Dawley rats,purified on the basis of their ability to adhere to the matrix,and expanded through their self-renewal.Two-step strategy was adopted with BMSCs induced into insulin-producing cells, After that,the cells were incubated with SPIO-PLL.Prussian blue stain was employed for identifying intracellular irons.Radioimmunology assay was used to measure the insulin secretion by the labeled and unlabeled cells,and later on underwent MR imaging with T_1WI、T_2WI、T_2*WI sequences,Results lntracytoplasmic nanoparticales were stained with Prussian blue possessing insulin-producing cells labeled with SPIO-PLL.The amount of insulin secreted by the labeled and unlabeled ceils had no statistical significant difference.The signal intensity of labeled cells decreased significantly on T_2*WI,as well as the stronger proportional variations for signal intensity.Conclusion Insulin-producing cells can be labelled effectively with SPIO-PLL and be imaged by 1.5 T MRI.(J Intervent Radiol,2007,16:104-108)
