Experimental research on treating hepatic carcinoma by arterial injection of iiposome mediated p53 genes
- VernacularTitle:经导管动脉注入脂质体介导的p53基因治疗肝癌的实验研究
- Author:
Guang-Yu ZHU
;
Qin LU
;
Gao-Jun TENG
;
Xiao-Ying WEI
;
Jin-He GUO
;
Hui YU
;
Gang DENG
;
Shi-Cheng HE
;
Wen FANG
;
Guozhao LI
;
- Publication Type:Journal Article
- Keywords:
p53 gene;
Liposome;
Transfection efficiency;
hepatic carcinoma;
Radiology,interventional
- From:
Journal of Interventional Radiology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the transfection and expression of p53 genes mediated by liposome and its feasibility in treatment of liver cancer by transcatheter arterial injection on rabbit VX2 hepatocarcinoma model.Methods pCMV-myc-p53 plasmids,LipofectAMINE and p53-LipofectAMINE complex were infused into tumor's feeding artery of rabbit VX2 hepatocarcinoma model,respectively,and then protein of cancer tissue was extracted,followed by measuring gene transfection and expression by western blot and immunohistochemistry,p53-LipofectAMINE complex in different doses were infused into tumor's feeding artery of rabbit VX2 hepatocarcinoma model with the gene transfection and expression detected by the same way.Results Liposome-mediated p53 gene injected through catheter could be successfully transfected and expressed in the cancer tissue of rabbit VX2 hepatocarcinoma model,with transfection efficiency higher than the gene delivery alone.The efficiency and the gene dose has dose-effect relationship.Condusions Treatment of liver cancer by transcatheter arterial injection of p53 genes mediated by liposome is a feasible and effective method,with wide prospect of application.(J Intervent Radiol,2007,16:109-114)
