Intermediate-conductance-Ca~(2+)-activated K~+ channels are overexpressed in endometrial cancer and involved in regulating proliferation of endometrial cancer cells
- VernacularTitle:钙激活性中电导钾离子通道在子宫内膜癌组织中的表达及其在细胞增殖中的作用
- Author:
Zhen-Hua WANG
;
You-Ji FENG
;
Min SU
;
Xiao-Fang YI
;
- Publication Type:Journal Article
- Keywords:
Endometrial neoplasms;
Potassium channels;
calcium-activated;
Cell division;
RNA;
small interfering
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of intermediate-conductance-Ca~(2+)-activated K~+ (IKCa1)channels in endometrial cancer and its role in regulating proliferation of endometrial cancer cells. Methods Western blot and RT-PCR were used to examine the expression of IKCa1 channels in 13 normal endometrial specimens and 25 endometrial cancer specimens;and RNA interference(RNAi),[ ~3H ] thymidine incorporation,and inhibitor of IKCa1 channel were used to explore the role of IKCa1 channels in regulation of proliferation of endometrial cancer cells HEC-1A.Results The expression rate and level of IKCa1 mRNA in endometrial carcinoma(84%,0.89?0.52)were higher than in normal endometria(8%, 0.14?0.12;P
