The chick embryo chorioaUantioc membrane as a model for in vivo research on anti-angiogenesis in endometriosis
- VernacularTitle:抗血管内皮生长因子抗体对异位子宫内膜鸡胚模型干预的研究
- Author:
Han-Bi WANG
;
Jin-Hua LENG
;
Lan ZHU
;
Zhu-Feng LIU
;
Da-Wei SUN
;
Jing-He LANG
;
- Publication Type:Journal Article
- Keywords:
Chick embryo;
Allantois;
Endometriosis;
Vascular endothelial growth factor A;
Antibodies
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the chick embryo chorioallantioc membrane(CAM)as a model for in vivo research on endometriosis.The model was used to investigate the mechanism of anti-vascular endothelial growth factor(VEGF)antibody for treatment of endometriosis.Methods Human endometrial fragments were explanted onto the CAM.Then anti-VEGF antibody was used for the endometriosis-like lesions after transplantation of human endometrial fragments.The CAM models were treated respectively as control groups and experimental groups.The terminal deoxynucleotidyl transferase-mediated biotin- deoxyuridine triphosphate(dUTP)nick end labeling(TUNEL),proliferating cell nuclear antigen(PCNA) and microvessel density(MVD)were used in vivo for analysis of anti-angiogenesis.Results The apoptosis intensity of anti-VEGF antibody treated groups(6.7?0,9,6.9?0.8)was significantly higher than that of the control groups(5.0?0.9,5.4?1.1;P
