The effects of low frequency ultrasound on the proliferation and apoptosis of smooth muscle cells in carotid atherosclerosis
- VernacularTitle:低频超声干预兔颈动脉粥样斑块的实验研究
- Author:
Yun-Bo CHEN
;
Chang-Qing LI
;
Qi SONG
;
Chuan-Shan XU
;
Le-Hua YU
;
- Publication Type:Journal Article
- Keywords:
Low frequency ultrasound;
Atherosclerosis;
Smooth muscle cells;
Apoptosis;
vascular smooth muscle cell proliferation
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of low frequency ultrasound (LFU) on the proliferation and apoptosis of smooth muscle cells (SMCs) in rabbits with carotid atherosclerosis(CA).Methods CA models were established in 30 New Zealand rabbits using a high fat diet and air-drying.They were randomly divided into a control group and four LFU groups:group A received 0.5 W/cm~2 LFU for 5 min/d,group B 0.5 W/cm~2 for 10 min/d, group C 1 W/cm~2,5 min/d,and group D 1 W/cm~2,10 min/d.The rabbits' carotid arteries were autopsied after 20 d of the LFU treatment.The expression of PCNA and TUNEL staining were used to explore the proliferation and apopto- sis of SMCs,and the proliferation rates (PRs) and apoptosis rates (ARs) were calculated.Results Compared with the control group,the PRs in groups B,C and D were significantly lower,while the ARs in groups B,C and D were significantly higher.There was no significant difference in ARs or PRs among groups B,C and D.Con- clusion LFU can induce SMC apoptosis and inhibit SMC proliferation in rabbits with CA.
