The effects of mild hypothermia on the expression of myeloperoxidase and cyclooxygenase 2 in rats after cerebral ischemia and reperfusion
- VernacularTitle:亚低温对大鼠局灶性脑缺血再灌注后白细胞髓过氧化酶、环氧合酶-2表达的影响
- Author:
Zhi-Chao LIU
;
Cheng-Yan LI
;
Qiu-Xiang CHENG
;
- Publication Type:Journal Article
- Keywords:
Mild hypothermia;
Brian ischemia;
Reperfusion;
Myeloperoxidase;
Cyclooxygenase
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of mild hypothermia ( MH ) on the expression of my- eloperoxidase(MPO) and cyclooxygenase 2 (COX 2) in rats after cerebral ischemia and reperfusion (CIR). Methods Forty-eight Wistar rats were randomly divided into four control groups (n=6 in each) and four MH groups (n=6 in each).CIR models were established by suture occlusion of the left middle cerebral artery.The rats in the MH groups,but not in the control groups,were treated with MH.Rats were killed at 4 h,8 h,12 h and 16 h after CIR.MPO expression was measured,along with the expression of COX 2 as measured by Western blot- ting and immunohistochemical methods.Results Compared with the control groups,MPO activity and the COX 2 expression in the cortex and striatum were significantly lower in all the MH groups at 4 h,8 h,12 h and 16 h after CIR.Conclusion MH treatment can protect neurons by decreasing MPO activity and COX 2 expression,allevia- ting inflammation and reducing secondary injuries after CIR.
