RP-HPLC Determination of Puerarin in Xinkeshu Tablet
- VernacularTitle:RP-HPLC法测定心可舒片中葛根素的含量
- Author:
Hua YIN
;
Qiang YUAN
;
Xiaoye DONG
;
Chengshuai XIANG
;
- Publication Type:Journal Article
- Keywords:
Xinkeshu Tablet;
Puerarin;
RP-HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a RP-HPLC method for determining the content of puerarin in Xinkeshu Tablets. Method The analysis was carried on a column of Zorbax Eclipse XDB-C_(18)(4.6 mm?150mm,5?m)at 25℃,with methanol-water-phosphoric acid(23:77:0.5)as mobile phase.The flow rate was 1.0 mL?min~(-1),and the detection wavelength was 250 nm.Result A good linearity was found in the range of 27.1～271.0 ng.The average recovery rate was 100.5 %,and RSD was 1.8%(n=5).Conclusion The method is simple,rapid,accurate,reproducible,and can be used to control the quality of Xinkeshu Tablets.
