Study on Extraction Process of Polysaccharide in Ophiopogon japonicus
- VernacularTitle:麦冬多糖的提取工艺考察
- Author:
Xiaoyan ZHANG
;
Xu ZHANG
;
Baochang CAI
;
Zhuoran CHEN
;
Bi SHI
;
- Publication Type:Journal Article
- Keywords:
Ophiopogon japonicus;
Polysaecharides;
Orthogonal design;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the extraction process and to establish a method for the content determination of the polysaccharides in Ophiopogonjaponicus.Methods With extracts rate of polysaccharide as the index,an orthogonal de- sign test was adopted to optimize the extraction process.And phenol-sulphuric acid colorimetric method was used to de- termine the content of polysaccharides in Ophiopogonjaponicus.Results The optimum extract conditions were as follows: refluxing twice with eight times amount of water,thirty minutes each time,and precipitation with 80 % alcohol.A good linearity of polysaccharides was in range of 0.02032～0.10008 mg(r=0.9998),and the average recovery rate was 102.41%,RSD was 1.78 %.Conclusion The polysaccharide can be fully extracted under these conditions.
