The expression of interferon-?in the peripheral blood and correlation with disease activity in systemic lupus erythematosus patients
- VernacularTitle:系统性红斑狼疮患者外周血IFN-?表达及其与疾病活动性的关系
- Author:
Shan DENG
;
Da-Wei HU
;
Yuan WANG
;
Nan SHEN
;
Yue-Ying GU
;
Chun-De BAO
;
Shun-Le CHEN
;
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus;
systemic;
Interferon-alpha;
SLEDAI score
- From:
Chinese Journal of Rheumatology
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mRNA and protein expression levels of interferon(IFN)-?in the peripheral blood of patients with systemic lupus erythematosus(SLE),to analyze the relationship between IFN-?and disease activity,and to evaluate the role of IFN-?in the pathogenesis of lupus.Methods SYBR green dyeⅠbased real-time quantatives PCR method was used to compare the mRNA expression levels of IFN-?in the peripheral blood leucocyte of SLE patients and healthy controls.Surum levels of IFN-?were measured with ELISA method.Results IFNA1 mRNA expression level in SLE patients(2.8?3.5)was signifi- cantly lower than that of normal controls(12.7?10.7,P=0.000).There was no significant difference between patients treated with glucocorticoid and those without in the expression level of IFNA1(P=0.549).Serum levels of IFN-?in SLE patients was significantly higher than that of normal controls(P=0.003).The SLEDAI score and anti-dsDNA antibody correlated positively,and complement components C3,C4 and leukocytes correlated negatively with serum concentration of IFN-?.IFN-?level correlated with the presence of fever and rash. Conclusion The close relationship between IFN-?serum level and disease activity in SLE patients suggests that IFN-?might be of importance in the disease process.
