Establishment of acute Respiratory distress syndrome model in beagle dogs

VernacularTitle:Beagle犬急性呼吸窘迫综合征模型的建立
Author: Yonghong DU ; Daoxin WANG ; Wenzhi CHEN ; Kequan LI ; Anning WEI ; Kai HU ; Jin BAI ; Zhibiao WANG ;
Publication Type:Journal Article
Keywords: Acute respiratory distress syndrome; Oleic acid; Animal model; Beagle dog
From: Journal of Chongqing Medical University 2003;0(05):-
CountryChina
Language:Chinese
Abstract: Objective:To explore the feasibility and stability of using oleic acid to establish acute respiratory distress syndrome(ARDS) model in Beagle dogs.Methods:A total of 25 Beagle dogs were injected oleic acid with a speed of 0.25?0.03ml/Kg through jugular vein after being anesthetized.The gas ventilation index,oxygen metabolism,histopathological changes,chest-ray scan and stability of each index after establishing the ARDS situation were continuously monitored and evaluated.Results:All 25 dogs were reached the diagnosis criteria of ARDS in 4.16?0.92 hours after injection of oleic acid,The arterial PaCO2 of the animals was 27.98?8.25mmHg,PaO2 was 65.40?11.48mmHg,and PaO2/FiO2 was 182.3?29.6.After mechanical ventilation treatment,PaO2/FiO2≤200.Pulmonary mesenchyme,and alveolus edema,bleeding,shrinkage and transparent formation were seen under microscope.There was darkness or coarse shadow in the lung by the X-ray scan.Conclusions:Through injection of oleic acid via jugular vein,a Beagle dog ARDS model may be quickly established with stable changes of hemodynamics,pulmonary mechanics and histology.